Cadherin 5 Antikörper (pTyr731)

Produktnummer ABIN7218860

Der Kaninchen Polyklonal Anti-Cadherin 5 Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, Cadherin 5 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Cadherin 5 Antikörper (pTyr731) (ABIN7218860)

Target: Cadherin 5 (CDH5)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cadherin 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cadherin 5 Antikörper

Bindungsspezifität: pTyr731

Verwendungszweck: VE-Cadherin (phospho Tyr731) Polyclonal Antibody

Spezifität: Phospho-VE-Cadherin (Y731) Polyclonal Antibody detects endogenous levels of VE-Cadherin protein only when phosphorylated at Y731.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human VE-Cadherin Phospho-Tyr731

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu Cadherin 5

Target: Cadherin 5 (CDH5)

Andere Bezeichnung: VE-Cadherin

Hintergrund: Rabbit Anti-VE-Cadherin (phospho Tyr731) Polyclonal Antibody,CDH5, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD antigen CD144,Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin-5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74 % homology.,Cadherin-5

Molekulargewicht: observerd band 130kDa

Gen-ID: 1003

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2