ACLY Antikörper

Produktnummer ABIN7213732

Der Kaninchen Polyklonal anti-ACLY Antikörper wird verwendet zum Nachweis von ACLY in Proben von Human, Maus, Ratte und Affe. Er wurde validiert für WB, ELISA und IF.

Kurzübersicht für ACLY Antikörper (ABIN7213732)

Target: ACLY (ATP Citrate Lyase (ACLY))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACLY Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-ACLY Antikörper

Verwendungszweck: Rabbit Anti-ATP-citrate synthase Polyclonal Antibody

Spezifität: ATP-citrate synthase Polyclonal Antibody detects endogenous levels of ATP-citrate synthase protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ATP-citrate synthase around the non-phosphorylation site of S455

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Andere Bezeichnung: ATP-citrate synthase

Hintergrund: ACLY, ATP-citrate synthase, ATP-citrate, pro-S-)-lyase, ACL, Citrate cleavage enzymeATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for ACLY.

Molekulargewicht: 120kD

Gen-ID: 47

UniProt: P53396

Pathways: Warburg Effekt