APAF1 Antikörper (AA 260-340)

Produktnummer ABIN7213614

Dieser Anti-APAF1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von APAF1 in WB, ELISA, IF und IHC (p). Geeignet für Human.

Kurzübersicht für APAF1 Antikörper (AA 260-340) (ABIN7213614)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-APAF1 Antikörper

Bindungsspezifität: AA 260-340

Verwendungszweck: Apaf-1-ALT Polyclonal Antibody

Spezifität: Apaf-1-ALT Polyclonal Antibody detects endogenous levels of Apaf-1-ALT protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Apaf-1-ALT at AA range: 260-340

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: Apaf-1-ALT

Hintergrund: Rabbit Anti-Apaf-1-ALT Polyclonal Antibody,APAF1-interacting protein, APIP, APIP2, CGI-29 protein, MMRP19, dJ179L10.2, likely ortholog of mouse monocyte macrophage 19,APAF1 encodes a cytoplasmic protein that initiates apoptosis. This protein (apoptotic peptidase activating factor 1) contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.,Apoptotic protease-activating factor 1

Gen-ID: 317

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity