alpha Actinin Antikörper

Produktnummer ABIN7212180

Der Maus Monoklonal anti-alpha Actinin Antikörper wird verwendet zum Nachweis von alpha Actinin in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für alpha Actinin Antikörper (ABIN7212180)

Target: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Actinin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: 4A12

Produktdetails anti-alpha Actinin Antikörper

Verwendungszweck: Mouse Anti-a-actinin Monoclonal Antibody (4A12)

Spezifität: A-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1.

Aufreinigung: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Recombinant Protein of a-actinin of ACTN1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:200-400,IF/ICC1:200-400,WB 1:500-2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu alpha Actinin

Target: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Andere Bezeichnung: a-actinin

Hintergrund: ACTN1Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN1 (actinin alpha 1) encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for ACTN1.

Molekulargewicht: 103kD

Gen-ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization, Negative Regulation of Transporter Activity