AFT1 Antikörper

Produktnummer ABIN7222640

Der Kaninchen Polyklonal anti-AFT1 Antikörper wird verwendet zum Nachweis von AFT1 in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für AFT1 Antikörper (ABIN7222640)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AFT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-AFT1 Antikörper

Verwendungszweck: Rabbit Anti-ATF-1 Polyclonal Antibody

Spezifität: ATF-1 Polyclonal Antibody detects endogenous levels of ATF-1 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ATF-1 around the non-phosphorylation site of S63

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Andere Bezeichnung: ATF-1

Hintergrund: ATF1, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36ATF1 encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of ATF1 and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. ATF1 has a pseudogene on chromosome 6.

Molekulargewicht: 29kD

Gen-ID: 466

UniProt: P18846

Pathways: Neurotrophin Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades