Actin, Alpha, gamma Antikörper

Produktnummer ABIN7223846

Der Kaninchen Polyklonal anti-Actin, Alpha, gamma Antikörper wird verwendet zum Nachweis von Actin, Alpha, gamma in Proben von Human, Maus und Ratte. Er wurde validiert für ELISA, IHC, IF und WB.

Kurzübersicht für Actin, Alpha, gamma Antikörper (ABIN7223846)

Target: Actin, Alpha, gamma

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Actin, Alpha, gamma Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB)

Produktdetails anti-Actin, Alpha, gamma Antikörper

Verwendungszweck: Rabbit Anti-Actin-α/γ Polyclonal Antibody

Spezifität: Actin-α/γ Polyclonal Antibody detects endogenous levels of Actin-α/γ protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Actin-alpha/gamma around the non-phosphorylation site of Y55/53

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Actin, Alpha, gamma

Target: Actin, Alpha, gamma

Andere Bezeichnung: Actin-alpha/gamma

Hintergrund: ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTG1, ACTB, ACTG, Actin, cytoplasmic 2, Gamma-actin, ACTG2, ACTA3, ACTL3, ACTSG, Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin,The product encoded by ACTA1 (actin, alpha 1, skeletal muscle) belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in ACTA1 cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Molekulargewicht: 45kD

Gen-ID: 58, 70, 71

UniProt: P68032, P63261, P63267, P68133