Topoisomerase II alpha Antikörper (AA 1100-1530)

Produktnummer ABIN7193564

Der Maus Monoklonal Anti-Topoisomerase II alpha-Antikörper wurde für WB, ELISA, FACS und ICC validiert. Er ist geeignet, Topoisomerase II alpha in Proben von Human und Ratte zu detektieren.

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1100-1530) (ABIN7193564)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 6C12G12

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1100-1530

Verwendungszweck: TOP2A Antibody

Aufreinigung: Purified antibody

Immunogen: Purified recombinant fragment of human TOP2A (AA: 1100-1530) expressed in E. Coli.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200-1/400

ICC: 1/100 - 1/500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A

Hintergrund:

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Molekulargewicht: 174.4 kDa

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases