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PD-L1 Antikörper (AA 19-238)

Name: PD-L1 Antikörper (AA 19-238)
Brand: antibodies-online
SKU: ABIN7164674
Price: 462 EUR
Availability: InStock

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PD-L1 in WB, FACS, IHC, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human.

Western Blot Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate All lanes: PD-L1 antibody at 1:2500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 33 kDa Observed band size: 55 kDa (PD-L1 Antikörper (AA 19-238))

Overlay histogram showing A549 cells stained with ABIN7164674 (red line) at 1:150. (PD-L1 Antikörper (AA 19-238))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate All lanes: PD-L1 antibody at 1:1000, 1:2000, 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 33 kDa Observed band size: 55 kDa (PD-L1 Antikörper (AA 19-238))

IHC image of ABIN7164674 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (PD-L1 Antikörper (AA 19-238))

IHC

IHC image of ABIN7164674 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. (PD-L1 Antikörper (AA 19-238))

IHC

Immunofluorescence staining of 293 cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 Antikörper (AA 19-238))

Immunofluorescence staining of A549 cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 Antikörper (AA 19-238))

Immunofluorescence staining of Hela cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 Antikörper (AA 19-238))

Overlay histogram showing 293 cells stained with ABIN7164674 (red line) at 1:150. (PD-L1 Antikörper (AA 19-238))

FACS

9 Abbildungen

Produktnummer ABIN7164674

Kurzübersicht für PD-L1 Antikörper (AA 19-238) (ABIN7164674)

Target

Alle PD-L1 Antikörper anzeigen

PD-L1 (CD274 (PD-L1))

Reaktivität

Human

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser PD-L1 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Klon

9B5F4

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Bindungsspezifität
72

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AA 19-238

Kreuzreaktivität

Human

Aufreinigung

Protein G purified

Immunogen

Recombinant Human Programmed cell death 1 ligand 1 protein (19-238AA)

Isotyp

IgG2b
anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 Reaktivität: Human WB, FACS, ELISA, IF Wirt: Ziege Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-153) antibody
PD-L1 Reaktivität: Human FACS, IHC, ELISA Wirt: Maus Monoclonal 6D9A2 unconjugated

anti-CD274 (PD-L1) (AA 39-191) antibody
PD-L1 Reaktivität: Human, Maus WB, FACS, IHC, ELISA, IF, Coat, StM Wirt: Maus Monoclonal PDL1-2746 unconjugated

anti-CD274 (PD-L1) (AA 48-290) antibody
PD-L1 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 34-241) antibody
PD-L1 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 19-238) antibody
PD-L1 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-153) antibody
PD-L1 Reaktivität: Human FACS, ELISA Wirt: Maus Monoclonal 7D2A10 unconjugated

anti-CD274 (PD-L1) (C-Term) antibody
KO Validated PD-L1 Reaktivität: Human WB, FACS, IF, ICC, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-234) antibody
PD-L1 Reaktivität: Human WB, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 201-290) antibody
PD-L1 Reaktivität: Maus, Ratte WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:100-1:300,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

PD-L1 (CD274 (PD-L1))

Andere Bezeichnung

PD-L1

Hintergrund

Background: Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077)
Aliases: Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274), CD274, B7H1 PDCD1L1 PDCD1LG1 PDL1

UniProt

Q9NZQ7

Pathways

Cancer Immune Checkpoints