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His Tag Antikörper

Name: His Tag Antikörper
Brand: antibodies-online
SKU: ABIN7131650
Price: 220 EUR
Availability: InStock

WB, ELISA Wirt: Maus Monoclonal 3G5H8 unconjugated

Western Blot Positive WB detected in: HIS fusion protein 1 at 20 ng, 10 ng, 5 ng, 2. (His Tag Antikörper)

Western Blot Positive WB detected in: HIS fusion protein 2 at 20 ng, 10 ng, 5 ng, 2. (His Tag Antikörper)

2 Abbildungen

Produktnummer ABIN7131650

Datenblatt (PDF)

Target Alle His Tag Antikörper anzeigen

His Tag

Reaktivität

Bitte anfragen
Wirt
86

53

11

6

5

4

1

1
Maus
Passende Sekundärantikörper
Klonalität
87

67

10
Monoklonal
Konjugat
70

23

14

11

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser His Tag Antikörper ist unkonjugiert
Applikation
131

84

34

26

24

15

14

14

13

13

12

7

6

6

5

4

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB), ELISA

Aufreinigung

Protein A purified

Immunogen

HHHHHH(6x His) synthetic peptide conjugate to KLH

Klon

3G5H8

Isotyp

IgG2a

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Applikationshinweise

Recommended dilution: WB:1:500-1:2000,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

His Tag

Andere Bezeichnung

6*His (His Tag Produkte)

Substanzklasse

Tag

Hintergrund

Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag