His Tag Antikörper

Produktnummer ABIN7131650

Dieses Maus Monoklonal-Antikörper erkennt spezifisch His Tag in WB und ELISA. Er zeigt eine Reaktivität gegenüber .

Kurzübersicht für His Tag Antikörper (ABIN7131650)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 3G5H8

Produktdetails anti-His Tag Antikörper

Aufreinigung: Protein A purified

Immunogen: HHHHHH(6x His) synthetic peptide conjugate to KLH

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Recommended dilution: WB:1:500-1:2000,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6*His

Substanzklasse: Tag

Hintergrund:

Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.

Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag