Rekombinanter RPA2 Antikörper (pThr21)

Produktnummer ABIN7127742

Der Kaninchen Monoklonal Anti-RPA2-Antikörper wurde für ELISA und IF validiert. Er ist geeignet, RPA2 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter RPA2 Antikörper (pThr21) (ABIN7127742)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser RPA2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF)

Klon: 3B2

Produktdetails anti-RPA2 Antikörper

Bindungsspezifität: pThr21

Kreuzreaktivität: Human

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-RPA2 (T21)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended dilution: IF:1:20-1:200,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: RPA2

Hintergrund:

Background: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Aliases: Replication protein A 32 kDa subunit, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34, RPA2, REPA2, RPA32, RPA34

UniProt: P15927

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA