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Rekombinanter POLR2A/RPB1 Antikörper (pSer5)

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch POLR2A/RPB1 in WB, IHC, IF und ELISA. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN7127726

Kurzübersicht für Rekombinanter POLR2A/RPB1 Antikörper (pSer5) (ABIN7127726)

Target

Alle POLR2A/RPB1 (POLR2A) Antikörper anzeigen
POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Antikörpertyp

Recombinant Antibody

Reaktivität

  • 97
  • 42
  • 41
  • 6
  • 6
  • 6
  • 6
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Human

Wirt

  • 76
  • 22
  • 1
  • 1
  • 1
Kaninchen

Klonalität

  • 52
  • 49
Monoklonal

Konjugat

  • 70
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 1
  • 1
Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation

  • 64
  • 35
  • 33
  • 31
  • 22
  • 10
  • 10
  • 10
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Klon

2H4
  • Bindungsspezifität

    • 20
    • 18
    • 11
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer5

    Kreuzreaktivität

    Human

    Aufreinigung

    Affinity-chromatography

    Immunogen

    A synthesized peptide derived from human Phospho-POLR2A (S5)

    Isotyp

    IgG
  • Applikationshinweise

    Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200,

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • Target

    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

    Andere Bezeichnung

    POLR2A

    Hintergrund

    Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity).

    Aliases: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, POLR2A, POLR2

    UniProt

    P24928

    Pathways

    Regulatorische RNA Pathways
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