Rekombinanter BUB1 Antikörper

Produktnummer ABIN7127373

Der Kaninchen Monoklonal Anti-BUB1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, BUB1 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter BUB1 Antikörper (ABIN7127373)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser BUB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 9C11

Produktdetails anti-BUB1 Antikörper

Kreuzreaktivität: Human

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Bub1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended dilution: WB:1:500-1:5000,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Andere Bezeichnung: BUB1

Hintergrund:

Background: Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Aliases: Mitotic checkpoint serine/threonine-protein kinase BUB1 (hBUB1) (EC 2.7.11.1) (BUB1A), BUB1, BUB1L

UniProt: O43683