KRT10, KRT13 Antikörper

Produktnummer ABIN7127244

Dieser Maus Monoklonal Antikörper detektiert spezifisch KRT10, KRT13 in FACS, IF, IHC und WB. Es zeigt Reaktivität gegenüber Proben von Cat und Human.

Kurzübersicht für KRT10, KRT13 Antikörper (ABIN7127244)

Target: KRT10, KRT13

Reaktivität: Cat, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Klon: DE-K13

Produktdetails

Verwendungszweck: Cytokeratin 10/13 Antibody

Spezifität: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Kreuzreaktivität (Details): Human and Cat.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: A431, HeLa or MCF7 cells. Human tonsil, esophageal or bladder carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: KRT10, KRT13

Andere Bezeichnung: KRT10 & KRT13

Hintergrund:

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT13, Type I keratin Ka13

A431, esophageal or bladder carcinoma., HeLa or MCF7 cells. Human tonsil

Molekulargewicht: 56.5kDa (CK10) & 53kDa (CK13)