KRT10, KRT13 Antikörper

Produktnummer ABIN6941317

Dieser Maus Monoklonal Antikörper detektiert spezifisch KRT10, KRT13 in FACS, IF, IHC und WB. Es zeigt Reaktivität gegenüber Proben von Cat und Human.

Kurzübersicht für KRT10, KRT13 Antikörper (ABIN6941317)

Target: KRT10, KRT13

Reaktivität: Cat, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Klon: DE-K13

Produktdetails

Verwendungszweck: Cytokeratin 10/13 Antibody

Spezifität: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.25-0.5 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: KRT10, KRT13

Andere Bezeichnung: KRT10 & KRT13

Hintergrund:

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT13, Type I keratin Ka13

A431, esophageal or bladder carcinoma., HeLa or MCF7 cells. Human tonsil

Molekulargewicht: 56.5kDa (CK10) & 53kDa (CK13)