Rekombinanter CD3 epsilon Antikörper (AA 100-200)

Produktnummer ABIN7127130

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch CD3 epsilon in IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CD3 epsilon Antikörper (AA 100-200) (ABIN7127130)

Target: CD3 epsilon (CD3E)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-CD3 epsilon Antikörper

Bindungsspezifität: AA 100-200

Spezifität: Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains, epsilon ( ), gamma (i³), delta ( L) and zeta ( A•), that assemble and function as three pairs of dimers ( i³, L, A• A•). The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR).CD3 antigen appearance at all stages of T cell development, and makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias).

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human CD3e fragment (around aa100-200) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, spleen or lymph node. Human peripheral blood or Jurkat cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E

Hintergrund: CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3e (T-Cell Marker)
Cellular localisation: Cell Surface. Cytoplasm.

Molekulargewicht: 20-25kDa

Gen-ID: 916, 3003

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway