HDAC3 Antikörper

Produktnummer ABIN7127118

Dieser Maus Monoklonal Antikörper detektiert spezifisch HDAC3 in WB, ELISA, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für HDAC3 Antikörper (ABIN7127118)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HDAC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: PCRP-HDAC3-3C9

Produktdetails anti-HDAC3 Antikörper

Verwendungszweck: Histone Deacetylase 1 (HDAC3) Antibody

Spezifität: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human HDAC3 protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Andere Bezeichnung: HDAC3

Hintergrund:

HD3, HDAC 3, HDAC3, HDAC3_HUMAN, Histone deacetylase 3, RPD3 2, RPD3, RPD3-2, SMAP45

HeLa or Jurkat cells.

Molekulargewicht: 49kDa

Gen-ID: 8841, 519632

UniProt: O15379

Pathways: Neurotrophin Signalübertragung, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development