CBFB Antikörper

Produktnummer ABIN7127112

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CBFB in WB, ELISA, IF, IP, FACS und Coat. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CBFB Antikörper (ABIN7127112)

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CBFB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Produktdetails anti-CBFB Antikörper

Spezifität: The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFA1 (core binding factor) and AML3 (acute myeloid leukemia), is composed of two subunits, i± and i², which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2i± subunits, PEBP2i±A, PEBP2i±B and PEBP2i±C, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory i² subunit, which facilitates DNA binding of the i± subunit. Both subunits are required for in vivo function, the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2i² is the target of chromosomal inversion 16 (p13,q22) with the smooth muscle Myosin heavy chain, producing a chimeric gene, PEBP2i²/CBFB-SMMHC, that is associated with human acute myeloid leukemia.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CBFB protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U87 or Jurkat cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Andere Bezeichnung: CBFB

Hintergrund: CBF b; CBFB; Core-binding factor, beta subunit (CBFB), transcript variant 2; PEBP2 beta; PEBP2B; Polyomavirus enhancer binding protein 2 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit,CBFB / PEBP2β
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.

Molekulargewicht: 22kDa

Gen-ID: 865, 519632

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha