CBFB Antikörper

Produktnummer ABIN7127112

Dieser Maus Monoklonal Antikörper detektiert spezifisch CBFB in WB, ELISA, IF, IP, FACS und Coat. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CBFB Antikörper (ABIN7127112)

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CBFB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Produktdetails anti-CBFB Antikörper

Spezifität: The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFA1 (core binding factor) and AML3 (acute myeloid leukemia), is composed of two subunits, i± and i², which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2i± subunits, PEBP2i±A, PEBP2i±B and PEBP2i±C, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory i² subunit, which facilitates DNA binding of the i± subunit. Both subunits are required for in vivo function, the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2i² is the target of chromosomal inversion 16 (p13,q22) with the smooth muscle Myosin heavy chain, producing a chimeric gene, PEBP2i²/CBFB-SMMHC, that is associated with human acute myeloid leukemia.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CBFB protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U87 or Jurkat cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Andere Bezeichnung: CBFB

Hintergrund: CBF b; CBFB; Core-binding factor, beta subunit (CBFB), transcript variant 2; PEBP2 beta; PEBP2B; Polyomavirus enhancer binding protein 2 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit,CBFB / PEBP2β
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.

Molekulargewicht: 22kDa

Gen-ID: 865, 519632

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha