Rekombinanter Vimentin Antikörper (AA 2-466)

Produktnummer ABIN7127051

Der Kaninchen Monoklonal anti-Vimentin Antikörper (Klon VIM-6430R) (ABIN7127051) detektiert spezifisch Vimentin in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Schwein, Rind (Kuh), Huhn, Hund, Cat und Pferd.

Kurzübersicht für Rekombinanter Vimentin Antikörper (AA 2-466) (ABIN7127051)

Target: Vimentin (VIM)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Schwein, Rind (Kuh), Huhn, Hund, Cat, Pferd

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: VIM-6430R

Produktdetails anti-Vimentin Antikörper

Bindungsspezifität: AA 2-466

Verwendungszweck: Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

Spezifität: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Kreuzreaktivität (Details): Human, Rat, Horse, Chicken, Cow, Cat, Dog, and Pig. Very broad species reactivity. Does not react with Mouse.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U-87, Raji, Jurkat or HeLa cells. Human lymph node or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: VIM

Hintergrund:

VIM

Raji, U-87, Jurkat or HeLa cells. Human lymph node or tonsil. A549, U-87, Human Skeletal muscle. Lung, Skin.

Molekulargewicht: 57-60kDa

Gen-ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose