Vinculin Antikörper

Produktnummer ABIN7127045

Dieses Anti-Vinculin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Vinculin in IF, FACS und IHC (f). Geeignet für Human.

Kurzübersicht für Vinculin Antikörper (ABIN7127045)

Target: Vinculin (VCL)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vinculin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Vinculin Antikörper

Spezifität: This antibody recognizes both Vinculin (125 kDa) and meta-Vinculin (135 kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and i±-Actinin. In addition, vinculin, Talin and i±-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. i±-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Kreuzreaktivität (Details): Human, Mouse, Rat, Cow, Pig, Rabbit, Frog, Fish and Bird.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Vinculin protein (VCL)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431, K562, NIH3T3, U-87, THP-1 cells. Bladder and Testis

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: VCL

Hintergrund: CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, VCL, VINC,Vinculin (Marker of Age-related Macular Degeneration)
Cellular localisation: Cytoplasmic

Molekulargewicht: 125-135kDa

Gen-ID: 7414, 643896

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2