ube3a Antikörper

Produktnummer ABIN7127039

Dieses Anti-ube3a-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ube3a in IF und FACS. Geeignet für Human.

Kurzübersicht für ube3a Antikörper (ABIN7127039)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ube3a Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-ube3a Antikörper

Spezifität: E6-associating protein (E6-AP), also designated ubiquitin protein ligase E3A(UBE3A), is a component of the ubiquitin-mediated proteolytic pathway thatselectively targets proteins for degradation by the 26S Proteasome. Ubiquitin(Ub) is directly conjugated to protein substrates by the transfer of Ub from anE2 ubiquitin conjugating enzyme to the target protein. This conjugation is facil-itated by the enzymatic activity of E3 ubiquitin ligase family members such asE6-AP. Several substrates of E6-AP have been identified and include the tumorsuppressor protein p53 and the mammalian homolog of Rad23, HHR23A.Previous studies have indicated that E6-AP associates with the humanpapillomavirus E6 oncogene, which forms a complex with p53 and therebypotentiates E6-AP mediated ubiquitination of p53. Genetic mutations thatimpair E6-AP activity result in the accumulation of p53 in the cytoplasm, andin many instances, these mutations are associated with the development ofthe rare neurodevelopmental disorder Angelman syndrome (AS), which ischaracterized by severe motor dysfunction and mental retardation.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human UBE3A protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Andere Bezeichnung: UBE3A

Hintergrund: ANCR, Angelman syndrome (AS), CTCL tumor antigen se37-2, E6AP ubiquitin protein ligase, EPVE6AP, FLJ26981, HECT-type ubiquitin transferase E3A, Human papilloma virus E6 associated protein Angelman syndrome, HPVE6A, NY REN 54 antigen, Oncogenic protein associated protein E6AP, Renal carcinoma antigen NY REN 54,UBE3A / E6-AP
Cellular localisation: Nucleus. Cytoplasm.

Molekulargewicht: 100kDa

Gen-ID: 7337, 598862

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway