Transglutaminase 2 Antikörper

Produktnummer ABIN7126998

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Transglutaminase 2 in WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Transglutaminase 2 Antikörper (ABIN7126998)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transglutaminase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Transglutaminase 2 Antikörper

Spezifität: Recognizes a 77-85 kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Kreuzreaktivität (Details): Human, Monkey, Rabbit, Mouse and Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human TGM2 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HUVEC cells. Endothelial cells in placenta, liver, brain, or breast. Smooth muscle cells of any origin (e.g. intestine)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Andere Bezeichnung: TGM2

Hintergrund: Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGase C, TGase H, TGase-2, TgaseII, TGC, TGM2, Tissue type transglutaminase, Transglutaminase 2 C polypeptide, Transglutaminase H, tTG, tTGas,Transglutaminase II (TGM2)
Cellular localisation: Cytoplasmic and cell surface

Molekulargewicht: 77-85kDa

Gen-ID: 7052, 517033

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling