SPARC Antikörper (AA 1-200)

Produktnummer ABIN7126971

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SPARC in WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SPARC Antikörper (AA 1-200) (ABIN7126971)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SPARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-SPARC Antikörper

Bindungsspezifität: AA 1-200

Spezifität: SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human placenta, lymph node or spleen. JEG3 or A549 cell lysates.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Andere Bezeichnung: SPARC

Hintergrund: Basement membrane protein 40, BM40, Cysteine rich protein, OI17, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and cysteine rich, Secreted protein acidic and rich in cysteine (SPARC),SPARC / Osteonectin
Cellular localisation: Secreted. Basement membrane.

Molekulargewicht: 38kDa

Gen-ID: 6678, 111779, 708558

UniProt: P09486

Pathways: Autophagie