Rekombinanter Smooth Muscle Actin Antikörper

Produktnummer ABIN7126869

Der Kaninchen Monoklonal Anti-Smooth Muscle Actin-Antikörper wurde für IHC (f) validiert. Er ist geeignet, Smooth Muscle Actin in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Smooth Muscle Actin Antikörper (ABIN7126869)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Smooth Muscle Actin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Smooth Muscle Actin Antikörper

Spezifität: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ACTA2 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Andere Bezeichnung: ACTA2

Hintergrund: ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha Cardiac Actin, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46,Actin, Smooth Muscle (Leiomyosarcoma Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 42kDa

Gen-ID: 59, 500483

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development