PKC iota Antikörper (AA 100-300)

Produktnummer ABIN7126834

Dieses Anti-PKC iota-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PKC iota in IHC (f). Geeignet für Human.

Kurzübersicht für PKC iota Antikörper (AA 100-300) (ABIN7126834)

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PKC iota Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-PKC iota Antikörper

Bindungsspezifität: AA 100-300

Spezifität: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (i±, i²I, i²II and i³) and novel (n) PKC isoforms ( L, , A•, , , l/i, m and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC L and are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Kreuzreaktivität (Details): Human, Cat and Dog.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa100-300) of human PRKCI protein (exact sequence is proprietary)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Brain, liver or placenta.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Andere Bezeichnung: PRKCI

Hintergrund: APKC lambda/iota, Atypical protein kinase C lambda/iota, Atypical protein kinase C-lambda/iota, nPKC-iota, nPKCiota, PKC lambda/iota, PKCI, PKCiota, PKClambda, PRKC iota, PRKC lambda/iota, PRKCI, Protein kinase C iota type,Protein Kinase C iota / lambda / PRKCI
Cellular localisation: Cytoplasm. Cell Membrane. Nucleus.

Molekulargewicht: 68kDa

Gen-ID: 5584, 478199

UniProt: P41743

Pathways: Neurotrophin Signalübertragung, Cell-Cell Junction Organization, Tube Formation