Rekombinanter CD31 Antikörper (AA 28-330)

Produktnummer ABIN7126783

Der Kaninchen Monoklonal anti-CD31 Antikörper (Klon C31-6446R) (ABIN7126783) detektiert spezifisch CD31 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für Rekombinanter CD31 Antikörper (AA 28-330) (ABIN7126783)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD31 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: C31-6446R

Produktdetails anti-CD31 Antikörper

Bindungsspezifität: AA 28-330

Verwendungszweck: Recombinant CD31 / PECAM-1 (Endothelial Cell Marker) Antibody

Spezifität: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Kreuzreaktivität (Details): Human. Weakly reacts with Rat.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (aa 28-330) of human CD31 expressed in E. Coli (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: THP-1 or Jurkat cells. Human tonsil or angiosarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: PECAM1

Hintergrund:

EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'

THP-1 or Jurkat cells. Human tonsil or angiosarcoma.

Molekulargewicht: ~100kDa (endothelium) and ~130kDa (platelets)

Gen-ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization