Rekombinanter PAX5 Antikörper (AA 235-382)

Produktnummer ABIN7126756

Der Maus Monoklonal anti-PAX5 Antikörper (Klon rPAX5-4228) (ABIN7126756) detektiert spezifisch PAX5 in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Hund.

Kurzübersicht für Rekombinanter PAX5 Antikörper (AA 235-382) (ABIN7126756)

Target: PAX5 (Paired Box 5 (PAX5))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAX5 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: RPAX5-4228

Produktdetails anti-PAX5 Antikörper

Bindungsspezifität: AA 235-382

Verwendungszweck: Recombinant PAX5 / BSAP (Early B-Cell Marker) Antibody

Spezifität: The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

Kreuzreaktivität (Details): Human. Dog

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary)

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Raji, DAUDI or Ramos cells. Human tonsil, spleen or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PAX5

Target: PAX5 (Paired Box 5 (PAX5))

Andere Bezeichnung: PAX5

Hintergrund:

B cell lineage specific activator, BSAP, EBB-1, KLP, Paired box 5

Daudi or Ramos cells. Human tonsil, Raji, Spleen or lymph node.

Molekulargewicht: 42kDa

Gen-ID: 5079, 654464

UniProt: Q02548