MITF Antikörper
-
- Target Alle MITF Antikörper anzeigen
- MITF (Microphthalmia-Associated Transcription Factor (MITF))
-
Reaktivität
- Human
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser MITF Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)
- Spezifität
- MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.
- Kreuzreaktivität (Details)
- Human. Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human MITF protein
- Isotyp
- IgG2b
- Top Product
- Discover our top product MITF Primärantikörper
-
-
- Applikationshinweise
-
Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Immunofluorescence (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells.Human melanoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
-
- Target
- MITF (Microphthalmia-Associated Transcription Factor (MITF))
- Andere Bezeichnung
- MITF (MITF Produkte)
- Synonyme
- MITF antikoerper, ws2a antikoerper, mitfa antikoerper, MITF-A antikoerper, LOC100313655 antikoerper, mitfb antikoerper, CMM8 antikoerper, MI antikoerper, WS2 antikoerper, WS2A antikoerper, bHLHe32 antikoerper, BCC2 antikoerper, Bhlhe32 antikoerper, Gsfbcc2 antikoerper, Vitiligo antikoerper, Wh antikoerper, bw antikoerper, mi antikoerper, vit antikoerper, MITF-H antikoerper, MITF-M antikoerper, CMI9 antikoerper, melanogenesis associated transcription factor antikoerper, microphthalmia-associated transcription factor antikoerper, microphthalmia-associated transcription factor S homeolog antikoerper, Microphthalmia antikoerper, MITF antikoerper, mitf antikoerper, LOC100313655 antikoerper, mitf.S antikoerper, LOC100534525 antikoerper, MICROP antikoerper, Mitf antikoerper
- Hintergrund
-
BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A,Microphthalmia Transcription Factor (MITF)
Cellular localisation: Nucleus. - Molekulargewicht
- 52-56kDa (doublet)
- Gen-ID
- 4286
- UniProt
- O75030
- Pathways
- Chromatin Binding
-