MITF Antikörper

Produktnummer ABIN7126695

Der Maus Monoklonal Anti-MITF-Antikörper wurde für WB, IF, FACS und IP validiert. Er ist geeignet, MITF in Proben von Human zu detektieren.

Kurzübersicht für MITF Antikörper (ABIN7126695)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MITF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Produktdetails anti-MITF Antikörper

Spezifität: MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.

Kreuzreaktivität (Details): Human. Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MITF protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Immunofluorescence (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells.Human melanoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung: MITF

Hintergrund: BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A,Microphthalmia Transcription Factor (MITF)
Cellular localisation: Nucleus.

Molekulargewicht: 52-56kDa (doublet)

Gen-ID: 4286

UniProt: O75030

Pathways: Chromatin Binding