MEF2D Antikörper

Produktnummer ABIN7126680

Der Maus Monoklonal Anti-MEF2D-Antikörper wurde für FACS, IP, IF und IHC (f) validiert. Er ist geeignet, MEF2D in Proben von Human zu detektieren.

Kurzübersicht für MEF2D Antikörper (ABIN7126680)

Target: MEF2D (Myocyte Enhancer Factor 2D (MEF2D))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MEF2D Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-MEF2D Antikörper

Spezifität: The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5 and MRF4, are one class of identified factors. A second family of DNA-binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.

Kreuzreaktivität (Details): Human. Predicted to react in Mouse and Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MEF2D protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MEF2D

Target: MEF2D (Myocyte Enhancer Factor 2D (MEF2D))

Andere Bezeichnung: MEF2D

Hintergrund: MADS box transcription factor 2 polypeptide D, Myocyte specific enhancer factor 2, polypeptide D,MEF2D / Myocyte enhancer factor 2D (Transcription Factor)
Cellular localisation: Nucleus. Translocated by HDAC4 to nuclear dots.

Molekulargewicht: 55.94kDa

Gen-ID: 4209, 314327

UniProt: Q14814