MCM2 Antikörper (AA 650-750)

Produktnummer ABIN7126677

Der Maus Monoklonal Anti-MCM2-Antikörper wurde für IHC (f) validiert. Er ist geeignet, MCM2 in Proben von Human zu detektieren.

Kurzübersicht für MCM2 Antikörper (AA 650-750) (ABIN7126677)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-MCM2 Antikörper

Bindungsspezifität: AA 650-750

Spezifität: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (aa 650-750) of human MCM2 protein (exact sequence is proprietary)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, Jurkat or Ramos cells. Human tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Andere Bezeichnung: MCM2

Hintergrund: BM28, CCNL1, CDC like 1, cdc19, CDCL1, Cell devision cycle like 1, Cyclin like 1, DNA replication licensing factor MCM2, KIAA0030, Minichromosome maintenance complex component 2, Minichromosome maintenance deficient 2 (mitotin), Minichromosome maintenance protein 2 homolog, Mitotin, Nuclear protein BM28,MCM2 (ProliferationMarker)
Cellular localisation: Nuclear

Molekulargewicht: 102kDa

Gen-ID: 4171, 477481

UniProt: P49736

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA