Rekombinanter MBP Antikörper (AA 150-250)

Produktnummer ABIN7126672

Der Maus Monoklonal anti-MBP Antikörper (Klon rMBP-4288) (ABIN7126672) detektiert spezifisch MBP in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Meerschweinchen und Hamster.

Kurzübersicht für Rekombinanter MBP Antikörper (AA 150-250) (ABIN7126672)

Target: MBP

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Meerschweinchen, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: RMBP-4288

Produktdetails anti-MBP Antikörper

Bindungsspezifität: AA 150-250

Verwendungszweck: Recombinant Myelin Basic Protein Antibody

Spezifität: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human brain or astrocytoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MBP

Target: MBP

Hintergrund:

GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP

Human brain or astrocytoma. Mouse Brain, Rat Brain, Guinea pig Brain or Hamster Brain

Molekulargewicht: 14-22kDa

Gen-ID: 4155, 551713

UniProt: P02686