MBP Antikörper (AA 119-131)

Produktnummer ABIN7126670

Dieser Maus Monoklonal Antikörper detektiert spezifisch MBP in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Meerschweinchen, Rind (Kuh), Hamster und Kaninchen.

Kurzübersicht für MBP Antikörper (AA 119-131) (ABIN7126670)

Target: MBP

Reaktivität: Human, Ratte, Maus, Meerschweinchen, Rind (Kuh), Hamster, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Klon: MBP-4272

Produktdetails anti-MBP Antikörper

Bindungsspezifität: AA 119-131

Verwendungszweck: Myelin Basic Protein Antibody

Spezifität: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4272 recognizes an epitope in the 119-131 region of MBP, useful in clinical diagnosis to detect MBP levels in human, rat and cow MBP.

Kreuzreaktivität (Details): Human, bovine and rat, reacts weakly with rabbit. Does not react with guinea pig.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recognizes bovine BMP, in the region of residues 119-131 (GAEGQRPGFGYGG).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U87 cells. Human brain or astrocytoma tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MBP

Target: MBP

Hintergrund:

GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP

U87 cells. Human brain or astrocytoma tissue. Mouse Brain, Rat Brain, Guinea pig Brain or Hamster Brain

Molekulargewicht: 14-22kDa

Gen-ID: 4155, 551713

UniProt: P02686