Leptin Receptor Antikörper (AA 335-425)

Produktnummer ABIN7126638

Der Maus Monoklonal Anti-Leptin Receptor-Antikörper wurde für IHC (f) validiert. Er ist geeignet, Leptin Receptor in Proben von Human zu detektieren.

Kurzübersicht für Leptin Receptor Antikörper (AA 335-425) (ABIN7126638)

Target: Leptin Receptor (LEPR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Leptin Receptor Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Leptin Receptor Antikörper

Bindungsspezifität: AA 335-425

Spezifität: Although there is substantial evidence that body weight is physiologically regulated, the molecular basis of obesity is unknown. Five single-gene mutations in mice that result in an obese phenotype have been identified. The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84 % identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin. The leptin receptor, designated Ob-R, has been shown to be a single membrane-spanning receptor that most resembles the gp130 signal transducing component of the IL-6, G-CSF and LIF receptor. Ob-R mRNA is expressed in the choroid plexus and hypothalamus.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa335-425) of human LEPR protein (exact sequence is proprietary)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 or HeLa cells. Human heart, small intestine or fetal liver.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Leptin Receptor

Target: Leptin Receptor (LEPR)

Andere Bezeichnung: LEPR

Hintergrund: Db, Fa, HuB219, LEP-R, LEPR, LEPRD, Leptin receptor, Leptin receptor fatty, Leptin receptor gene related protein, Leptin receptor precursor, OB R gene related protein (OB-RGRP), OB receptor (OBR / OB-R), obl,Leptin Receptor (LEPR) / Ob-R / CD295
Cellular localisation: Secreted. Cell surface.

Molekulargewicht: 100kDa / 125kDa (short / long form)

Gen-ID: 3953

UniProt: P48357

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour