IL-7 Antikörper (AA 27-177)

Produktnummer ABIN7126578

Der Maus Monoklonal Anti-IL-7-Antikörper wurde für IF, FACS und IHC (f) validiert. Er ist geeignet, IL-7 in Proben von Human zu detektieren.

Kurzübersicht für IL-7 Antikörper (AA 27-177) (ABIN7126578)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-7 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-IL-7 Antikörper

Bindungsspezifität: AA 27-177

Spezifität: Interleukin-7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor specific chain and the IL-2 receptor i³ chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of Insulin receptor substrate-1 (IRS-1) and Insulin receptor substrate-2 (IRS-2), originally called 4PS.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 27-177) corresponding to mature protein of human IL7(exact sequence is proprietary)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human thymus or tonsil tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: IL7

Hintergrund: IL 7, IL-7, Il7, Interleukin 7, Interleukin-7, LP-1, Lymphopoietin 1,Interleukin-7 (IL-7)
Cellular localisation: Secreted.

Molekulargewicht: 25kDa

Gen-ID: 3574, 591873

Pathways: JAK-STAT Signalweg