Rekombinanter PD-L1 Antikörper (AA 39-191)

Produktnummer ABIN7126487

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PD-L1 in WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter PD-L1 Antikörper (AA 39-191) (ABIN7126487)

Target: PD-L1 (CD274 (PD-L1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PD-L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-PD-L1 Antikörper

Bindungsspezifität: AA 39-191

Spezifität: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: NIH3T3, PC3 or MCF-7 cells. Human placenta, spleen or tonsil tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu PD-L1

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: CD274

Hintergrund: B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1,PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)
Cellular localisation: Cell Surface and Cytoplasmic

Molekulargewicht: 37-50kDa

Gen-ID: 29126, 521989

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints