GCLM Antikörper (Regulatory Subunit)

Produktnummer ABIN7126478

Dieser Maus Monoklonal Antikörper detektiert spezifisch GCLM in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für GCLM Antikörper (Regulatory Subunit) (ABIN7126478)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GCLM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: CPTC-GCLM-1

Produktdetails anti-GCLM Antikörper

Bindungsspezifität: Regulatory Subunit

Verwendungszweck: Glutamate-Cysteine Ligase Regulatory Subunit Antibody

Spezifität: GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length GCLM protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Andere Bezeichnung: GCLM

Hintergrund:

GCLM Antibody, Gamma-ECS regulatory subunit Antibody, GLCLR Antibody, GCS light chain Antibody, GSC light chain Antibody

A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.

Molekulargewicht: 31kDa

Gen-ID: 2730, 315562

UniProt: P48507