CD15 Antikörper

Produktnummer ABIN7126452

Der Maus Monoklonal Anti-CD15-Antikörper wurde für IF und IHC (f) validiert. Er ist geeignet, CD15 in Proben von Human zu detektieren.

Kurzübersicht für CD15 Antikörper (ABIN7126452)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD15 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-CD15 Antikörper

Spezifität: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95 % of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified neutrophils from normal human peripheral blood

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U937 cells. Reed-Sternberg's cells in Hodgkin's lymphoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Andere Bezeichnung: FUT4

Hintergrund: 3 Fucosyl N Acetyl Lactosamine, Alpha (1,3) Fucosyltransferase, Alpha 13 fucosyltransferase FucT, ELAM Ligand Fucosyltransferase, ELFT, FCT3A, Fuc-TIV, Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific, Fucosyltransferase 4, Galactoside 3 L Fucosyltransferase, Lewis X, LeX, SSEA1, Stage Specific Embryonic Antigen 1,CD15 / FUT4 (Reed-Sternberg Cell Marker)
Cellular localisation: Cell surface and granular paranuclear

Molekulargewicht: ~220kDa

Gen-ID: 2526, 654379

UniProt: P22083