JAZF1 Antikörper (AA 1-130)

Produktnummer ABIN7126392

Produktdetails anti-JAZF1 Antikörper

Target: JAZF1 (JAZF Zinc Finger 1 (JAZF1))

Bindungsspezifität: AA 1-130

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser JAZF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)

Spezifität: JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.

Kreuzreaktivität (Details): Human. Predicted to react in Mouse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or U87 cells. Ubiquitous tissue expression.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu JAZF1

Target: JAZF1 (JAZF Zinc Finger 1 (JAZF1))

Andere Bezeichnung: JAZF1 (JAZF1 Produkte)

Synonyme: TIP27 antikoerper, ZNF802 antikoerper, JAZF1 antikoerper, AI591476 antikoerper, C820002C15 antikoerper, Jaz1 antikoerper, Tip27 antikoerper, zgc:103778 antikoerper, JAZF zinc finger 1 antikoerper, JAZF zinc finger 1b antikoerper, JAZF1 antikoerper, Jazf1 antikoerper, jazf1b antikoerper

Hintergrund: DKFZp761K2222, TIP27, ZNF802,JAZF1 (Transcription Corepressor)
Cellular localisation: Nucleus.

Molekulargewicht: 27.08kDa

Gen-ID: 221895, 368944

UniProt: Q86VZ6