EIF4E Antikörper
Kurzübersicht für EIF4E Antikörper (ABIN7126325)
Target
Alle EIF4E Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA cap during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.
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Kreuzreaktivität (Details)
- Human.
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Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogen
- Recombinant full-length human EIF4E protein
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Isotyp
- IgG2a
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Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, Raji or U87 cells.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Konservierungsmittel
- Azide free
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Haltbarkeit
- 24 months
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- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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Andere Bezeichnung
- EIF4E
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Hintergrund
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AUTS19, CBP, eIF-4F 25 kDa subunit, eIF4E, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E like 1, Messanger RNA (mRNA) Cap Binding Protein eIF 4E,EIF4E
Cellular localisation: Cytoplasm. -
Molekulargewicht
- 25.1kDa
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Gen-ID
- 1977, 249718
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UniProt
- P06730
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Pathways
- BCR Signaling
Target
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