Reelin Antikörper (AA 1796-2115)

Produktnummer ABIN7126322

Dieser Maus Monoklonal Antikörper detektiert spezifisch Reelin in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Nagetiere.

Kurzübersicht für Reelin Antikörper (AA 1796-2115) (ABIN7126322)

Target: Reelin (RELN)

Reaktivität: Human, Maus, Nagetiere

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Reelin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Klon: RELN-4498

Produktdetails anti-Reelin Antikörper

Bindungsspezifität: AA 1796-2115

Verwendungszweck: Reelin Antibody

Spezifität: Reelin (or Reln) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. Reelin was shown to be mutated in reeler mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. Reelin protein expression is complex and changes throughout development. Reelin appears to function upstream of Dab1, in a signaling pathway that controls cell positioning in the developing brain, and is also thought to be a direct effector of the neurotrophin BDNF.

Kreuzreaktivität (Details): Human, Mouse and Rodent.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 1796-2115) of human RELN protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human or mouse brain or CNS.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Reelin

Target: Reelin (RELN)

Andere Bezeichnung: RELN

Hintergrund:

LIS2, PRO1598, Reeler, Reelin, RELN, RELN_HUMAN, RL

Human or mouse brain or CNS.

Molekulargewicht: 387.5kDa

Gen-ID: 19699, 655654

UniProt: P78509

Pathways: Synaptic Membrane