EIF2S1 Antikörper

Produktnummer ABIN7126321

Dieses Anti-EIF2S1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von EIF2S1 in WB, IF und FACS. Geeignet für Human.

Kurzübersicht für EIF2S1 Antikörper (ABIN7126321)

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF2S1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-EIF2S1 Antikörper

Spezifität: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2a, eIF2band eIF2g (eukaryotic translation initiation factor 2 a, band g, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2a, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2a controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2a is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

Kreuzreaktivität (Details): Human. Predicted to work in Rat and Zebrafish.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human EIF2S1 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF7 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Andere Bezeichnung: EIF2S1

Hintergrund: Eukaryotic translation initiation factor 2 subunit 1 alpha 35 kDa (EIF2S1), EIF-2alpha, EIF2, EIF2A,EIF2S1 / EIF-2 alpha (EIF2A)
Cellular localisation: Nucleus.

Molekulargewicht: 36kDa

Gen-ID: 1965, 151777

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C