Rekombinanter Decorin Antikörper (AA 212-336)

Produktnummer ABIN7126285

Dieses Anti-Decorin-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von Decorin in IHC und IHC (f). Geeignet für Human.

Kurzübersicht für Rekombinanter Decorin Antikörper (AA 212-336) (ABIN7126285)

Target: Decorin (DCN)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Decorin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Decorin Antikörper

Bindungsspezifität: AA 212-336

Spezifität: Decorin is a small leucine-rich proteoglycan (SLRP) family member that consists of a glycosaminoglycan chain-containing core protein. The core protein contains ten leucine rich repeats that contain sites for glycosylation, flanked by disulfide bond stabilizing loops. Decorin binds to Collagen Type I, II and IV in vivo and promotes the formation of fibers with variations in stability and solubility. The Decorin core protein binds to growth factors, intercellular matrix molecules, such as Fibronectin and Thrombospondin, and to the Decorin endocytosis receptor. Decorin binds to and inhibits TGFi² and is a direct or indirect negative modulator of TGFi² synthesis. Inhibition of Decorin core protein gene expression by the combination of IFN-i³ and TNFi± may contribute to cartilage destruction that is characteristic of inflammatory joint diseases. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Decorin protein fragment (around aa212-336) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human prostate or skin tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Decorin

Target: Decorin (DCN)

Andere Bezeichnung: DCN

Hintergrund: Bone proteoglycan II, CSCD, Dermatan sulphate proteoglycans II (DSPG2), PG40, PGII, PGS2, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B,Decorin
Cellular localisation: Secreted.

Molekulargewicht: 43kDa

Gen-ID: 1634, 728830

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process