Decorin Antikörper

Produktnummer ABIN7126284

Der Maus Monoklonal anti-Decorin Antikörper wird verwendet zum Nachweis von Decorin in Proben von Human, Maus, Rind (Kuh), Hund, Schwein, Kaninchen, Pferd, Huhn und Elch. Er wurde validiert für WB, IHC, IF und FACS.

Kurzübersicht für Decorin Antikörper (ABIN7126284)

Target: Decorin (DCN)

Reaktivität: Human, Maus, Rind (Kuh), Hund, Schwein, Kaninchen, Pferd, Huhn, Elch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Decorin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: DCN-6289

Produktdetails anti-Decorin Antikörper

Verwendungszweck: Decorin / PGS2 Antibody

Spezifität: Decorin is a small leucine-rich proteoglycan (SLRP) family member that consists of a glycosaminoglycan chain-containing core protein. The core protein contains ten leucine rich repeats that contain sites for glycosylation, flanked by disulfide bond stabilizing loops. Decorin binds to Collagen Type I, II and IV in vivo and promotes the formation of fibers with variations in stability and solubility. The Decorin core protein binds to growth factors, intercellular matrix molecules, such as Fibronectin and Thrombospondin, and to the Decorin endocytosis receptor. Decorin binds to and inhibits TGF æ and is a direct or indirect negative modulator of TGF æ synthesis. Inhibition of Decorin core protein gene expression by the combination of IFN-Á and TNF°™ may contribute to cartilage destruction that is characteristic of inflammatory joint diseases. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope.

Kreuzreaktivität (Details): Human, Bovine, Canine, Chick, Elk, Equine, Mouse, Porcine and Rabbit.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length DCN protein

Isotyp: IgG1, lambda

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human prostate or skin tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Decorin

Target: Decorin (DCN)

Andere Bezeichnung: DCN

Hintergrund:

Bone proteoglycan II, CSCD, Dermatan sulphate proteoglycans II (DSPG2), PG40, PGII, PGS2, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B

Prostate or skin tissue.

Molekulargewicht: 43kDa

Gen-ID: 1634, 728830

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process