UBE2C Antikörper

Produktnummer ABIN7126211

Dieses Maus Monoklonal-Antikörper erkennt spezifisch UBE2C in ELISA, Coat und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für UBE2C Antikörper (ABIN7126211)

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UBE2C Antikörper ist unkonjugiert

Applikation: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-UBE2C Antikörper

Spezifität: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ubconjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.

Kreuzreaktivität (Details): Human

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length UBE2C protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, Jurkat or A431 cells. Human tonsil or placenta tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu UBE2C

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Andere Bezeichnung: UBE2C

Hintergrund: Cyclin selective ubiquitin carrier protein, mitotic specific ubiquitin conjugating enzyme, UB E2C, UBCH10, Ubiquitin protein ligase C,Ubiquitin-conjugating Enzyme E2C / UBCH10
Cellular localisation: Cytoplasm.

Molekulargewicht: 20kDa

Gen-ID: 11065, 93002

UniProt: O00762

Pathways: Ubiquitin Proteasome Pathway