Rekombinanter Podoplanin Antikörper (AA 1-100)

Produktnummer ABIN7126197

Der Kaninchen Monoklonal Anti-Podoplanin-Antikörper wurde für ELISA, IF, IHC (f) und Coat validiert. Er ist geeignet, Podoplanin in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Podoplanin Antikörper (AA 1-100) (ABIN7126197)

Target: Podoplanin (PDPN)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Podoplanin Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Produktdetails anti-Podoplanin Antikörper

Bindungsspezifität: AA 1-100

Spezifität: It recognizes a muco-protein of 38-43 kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues within aa1-100 of Podoplanin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, cervical or lung carcinoma tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Podoplanin

Target: Podoplanin (PDPN)

Andere Bezeichnung: PDPN

Hintergrund: Aggrus, Glycoprotein 36 KD, Glycoprotein 36, gp36, GP38, GP40, HT1A1, hT1alpha1, hT1alpha2, Lung type I cell membrane associated glycoprotein, Lung type I cell membrane associated glycoprotein T1A 2, OTS8,Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker)
Cellular localisation: Cell Surface

Molekulargewicht: 17kDa

Gen-ID: 10630, 468675

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport