HDAC6 Antikörper

Produktnummer ABIN7504168

Der Maus Monoklonal Anti-HDAC6-Antikörper wurde für IF, IP und FACS validiert. Er ist geeignet, HDAC6 in Proben von Human zu detektieren.

Kurzübersicht für HDAC6 Antikörper (ABIN7504168)

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HDAC6 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Produktdetails anti-HDAC6 Antikörper

Spezifität: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.This enzyme deacetylates lysine residues in histones H2A, H2B, H3 and H4.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human HDAC6 protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Positive Control: A549 or HeLa cells.

Known Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu HDAC6

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Andere Bezeichnung: HDAC6

Hintergrund: CPBHM, HDAC6, Histone deacetylase 6 (HD6), Histone deacetylase 6, JM21, PPP1R90, Protein phosphatase 1 regulatory subunit 90,HDAC6
Cellular localisation: Nuclear

Molekulargewicht: 160kDa

Gen-ID: 10013, 6764

UniProt: Q9UBN7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling