ZCCHC11 Antikörper

Produktnummer ABIN7121051

Der Kaninchen Polyklonal anti-ZCCHC11 Antikörper wird verwendet zum Nachweis von ZCCHC11 in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IF und IP.

Kurzübersicht für ZCCHC11 Antikörper (ABIN7121051)

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZCCHC11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-ZCCHC11 Antikörper

Verwendungszweck: TUT4 antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: zinc finger, CCHC domain containing 11

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:200-1:2000, IP: 1:500-1:5000, IF: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu ZCCHC11

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Andere Bezeichnung: TUT4

Hintergrund:

Synonyms: Terminal uridylyltransferase 4 (TUTase 4)|Zinc finger CCHC domain-containing protein 11|TUT4|KIAA0191|ZCCHC11

Background: Uridylyltransferase that mediates the terminal uridylation of mRNAs with short(less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay(PubMed:25480299). Involved in microRNA(miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7(pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem(ES) cell pluripotency(By similarity). Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression(By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.

Molekulargewicht: 185 kDa

Gen-ID: 23318

UniProt: Q5TAX3

Pathways: Stem Cell Maintenance