GPR177/WLS Antikörper (AA 43-234)

Produktnummer ABIN7120857

Dieses Anti-GPR177/WLS-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GPR177/WLS in ELISA, WB, IHC und IP. Geeignet für Human, Maus und Ratte.

Kurzübersicht für GPR177/WLS Antikörper (AA 43-234) (ABIN7120857)

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR177/WLS Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-GPR177/WLS Antikörper

Bindungsspezifität: AA 43-234

Verwendungszweck: WLS antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 43-234aa

Immunogen: G protein-coupled receptor 177

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:1000, IP: 1:500-1:1000, IHC: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu GPR177/WLS

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Andere Bezeichnung: WLS

Hintergrund:

Synonyms: Protein wntless homolog|Integral membrane protein GPR177|Protein evenness interrupted homolog (EVI)|Putative NF-kappa-B-activating protein 373|WLS|C1orf139|GPR177

Background: GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).

Molekulargewicht: 46-62 kDa

Gen-ID: 79971

UniProt: Q5T9L3

Pathways: WNT Signalweg