VRK2 Antikörper

Produktnummer ABIN7120700

Der Kaninchen Polyklonal Anti-VRK2-Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, VRK2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für VRK2 Antikörper (ABIN7120700)

Target: VRK2 (Vaccinia Related Kinase 2 (VRK2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VRK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-VRK2 Antikörper

Verwendungszweck: VRK2 antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: vaccinia related kinase 2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:5000,IP: 1:200-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu VRK2

Target: VRK2 (Vaccinia Related Kinase 2 (VRK2))

Andere Bezeichnung: VRK2

Hintergrund:

Synonyms: Serine/threonine-protein kinase VRK2|Vaccinia-related kinase 2|VRK2

Background: Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta(IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase(MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant. Isoform 2 phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.

Molekulargewicht: 67 kDa

Gen-ID: 7444

UniProt: Q86Y07